| 名稱 | GLP1R/CRE/Luc/HEK293 |
| 型號(hào) | CBP71117 |
| 報(bào)價(jià) |  |
| 特點(diǎn) | GLP1R/CRE/Luc HEK293 凍存條件：90% FBS+10% DMSO |
產(chǎn)品搜索
相關(guān)文章
- NK2R細(xì)胞篩選模型
- 科學(xué)家發(fā)現(xiàn)10個(gè)新的智力障礙易感基因
- 慢病毒整合位點(diǎn)標(biāo)準(zhǔn)品強(qiáng)勢(shì)來(lái)襲
- 腫瘤細(xì)胞培養(yǎng)實(shí)驗(yàn)的主要用途
- 有關(guān)腫瘤細(xì)胞的基因突變研究講解
- 泛腫瘤大panel質(zhì)控品升級(jí)，免費(fèi)試用申請(qǐng)中
- ATCC細(xì)胞的實(shí)驗(yàn)要點(diǎn)及說(shuō)明
- CYP2C9基因多態(tài)性用藥指導(dǎo)標(biāo)準(zhǔn)品
- MTHFR基因多態(tài)性用藥指導(dǎo)標(biāo)準(zhǔn)品
- CD27細(xì)胞篩選模型
聯(lián)系我們
聯(lián)系人：蔣經(jīng)理
電話：4008750250
號(hào)碼：
手機(jī)：18066071954
地址：南京市棲霞區(qū)緯地路9號(hào)
Email： zhangxiangwen@cobioer.com
電話：4008750250
號(hào)碼：
手機(jī)：18066071954
地址：南京市棲霞區(qū)緯地路9號(hào)
Email： zhangxiangwen@cobioer.com
產(chǎn)品展示 / PRODUCTS
藥靶細(xì)胞株 > GPCR細(xì)胞株 > CBP71117GLP1R/CRE/Luc/HEK293
- 詳細(xì)內(nèi)容
| CBP71117 | |
| I. Background | |
GLP1R binds specifically the glucagon-like peptide-1 (GLP1) and has much lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon. GLP1R is known to be expressed in pancreatic beta cells. Activated GLP1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis and release of insulin. Consequently, GLP1R has been suggested as a potential target for the treatment of diabetes. GLP1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice which over express GLP1R display improved memory and learning. | |
| II. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | Genbank Accession Number NM_002062; no expressed tags |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | GLP-1 |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+400ug/ml G418 |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): | Functional assay for GLP1R receptor |
| Transducer: | Gs |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅳ.Representative Data | |
  | |
Figure 1. GLP-1 (7-37)-induced concentration-dependent stimulation of intracellular luciferase in HEK293/GLP1R/Luc and HEK293 cells. After stimulation with GLP1 receptor agonist, GLP-1 (7-37), the cells are checked with BrightGlo™Luciferase Assay System and the relative luminescence units (RLU) of luciferase were recorded on a FlexStation. The RLU were plotted against the log of the cumulative doses (10 -fold dilution) of GLP-1 (7-37) (Mean ± SD, n = 2). The EC50 of GLP-1 (7-37) co-expressing with Luc in HEK293 cells was 4.91 nM. | |
會(huì)員_a.png)